Aquilos 2

Dedicated cryo-FIB microscope to prepare cryo-lamellas for cryo-electron tomography

• Status: Active
• Country: USA
• Electron gun type : Schottky FEG
• Standard detectors : T1, T2, ETD, ICE
• Optional detectors : Optional acoustic enclosure, EasyLift, iFLM
• Cryo : Yes
• FIB: Yes
• Additional analytical capabilities (EDS/EDX ; EELS; SAED;NBD; others) : EDS, cryo-lift-out, AutoScript
• Electron optics : NICol UHR non-immersion field emission-SEM column
• Resolution : 1.6 nm at 30 kV; 2.6 nm at 2 kV (RT); 6.0 nm at 2 kV (cryo)
• Electron beam resolution : 1.6 nm at 30 kV; 2.6 nm at 2 kV (RT); 6.0 nm at 2 kV (cryo)
• Beam current : 1.5 pA to 400 nA
• Accelerating voltage : 200 V – 30 kV
• Image parameters : 6144×4096 px; 25 ns to 25 ms/pixel
• Camera details : IR camera; Nav-Cam
• Stage parameters : 110×110 mm eucentric stage; Tilt: -15° to +55°
• Holder Types : Cryo-FIB AutoGrids, Leica CLEM, pin mount stubs
• Cooling system : LN2 Dewar, flow controller, exchanger
• Vacuum system : Oil-free; <4e⁻⁴ Pa (RT), <8e⁻⁵ Pa (cryo)
• Environmental requirements : Optional acoustic enclosure
• Vibration, Magnetic, Noise tolerance : Seismic restraint kit